Brucella spp. are the causative agents of brucellosis, which is among the most common zooneses globally. The mechanisms by which Brucella spp. maintain long-term interactions with their mammalian hosts are not well understood. The CCFA Brucella abortus research project led by Dr. Sean Crosson of the University of Chicago is elucidating the biochemical and regulatory functions of uncharacterized open reading frames (ORFs) and putative small non-coding RNAs that have been implicated in long-term mammalian infection. This project is directed toward the long-range goal of understanding the molecular mechanism of B. abortus general stress signaling, and the relationship between B. abortus stress physiology and chronic brucellosis.